abstract：:Two polypeptides (designated DTX-A and DTX-B) were purified from crude snake venom of Dendroaspis angusticeps using gel filtration, cation exchange column chromatography and cation exchange high performance liquid chromatography, and their blocking actions of K+ channels were investigated in rat brain synaptosomes. Bo...

journal_title：Neurochemistry international

authors： Chaki S,Muramatsu M,Ushiyama Y,Otomo S

更新日期：1992-06-01 00:00:00

abstract：:Insulin receptors from rat brain regions were studied for insulin binding and receptor associated kinase activity, in alloxan induced short-term and long-term diabetes, and insulin induced hypoglycemia. Insulin receptor activity was assessed by [125I]insulin binding, and basal as well as insulin stimulated kinase acti...

journal_title：Neurochemistry international

authors： Gupta G,Azam M,Baquer NZ

更新日期：1992-06-01 00:00:00

abstract：:The disposition of labelled [3H]GM1lactone, the inner ester of ganglioside GM1, was studied in the rat. After i.v. administration [3H]GM1lactone was quickly converted to its corresponding open form most likely by plasma esterases, and then displayed a pharmacokinetic profile identical to [3H]GM1. Following intramuscul...

journal_title：Neurochemistry international

authors： Bellato P,Milan F,Facchinetti E,Toffano G

更新日期：1992-04-01 00:00:00

abstract：:D1 dopamine receptors have been localized using a radioactive form of a new specific antagonist, [3H]SCH39166. This compound has been shown, in in vitro binding studies, to be highly selective for the D1 receptor subtype; more so than its predecessor, [3H]SCH23390. These ligand binds saturably, reversibly and with hig...

journal_title：Neurochemistry international

authors： Wamsley JK,Alburges ME,McQuade RD,Hunt M

更新日期：1992-03-01 00:00:00

abstract：:The present study was designed to investigate the turnover rates of D1 dopamine receptors in the brain and retina of adult and aged rats. To this aim, we monitored the increase in the density of 3H-SCH 23390 binding sites after the administration of the irreversible antagonist N-ethoxycarbonyl-2-ethoxy-1,2 dihydroquin...

journal_title：Neurochemistry international

authors： Giorgi O,Pibiri MG,Dal Toso R,Toffano G,Biggio G

更新日期：1992-03-01 00:00:00

abstract：:Amiloride, a potassium sparing diuretic, inhibits adenosine A1 receptor-radioligand binding in calf and rat brain membranes in the low micromolar range. The drug interacted with the A1 receptor in a manner different from classical A1 ligands, but structure-activity relationship studies indicated that this inhibitory e...

journal_title：Neurochemistry international

authors： Garritsen A,Beukers MW,IJzerman AP,Cragoe EJ Jr,Soudijn W

更新日期：1992-02-01 00:00:00

abstract：:Quantitative autoradiography analysis of neurotensin (NT) and somatostatin (SS) binding sites was performed on coronal sections of the medulla oblongata from 2 fetuses, 6 controls and 7 victims of Sudden Infant Death Syndrome (SIDS). Throughout the first postnatal year, mean SS binding site density was similar in cont...

journal_title：Neurochemistry international

authors： Chigr F,Jordan D,Najimi M,Denoroy L,Sarrieau A,de Broca A,Rostene W,Epelbaum J,Kopp N

更新日期：1992-01-01 00:00:00

abstract：:The active fraction, isolated and partially purified from the crude venom of the marine snail Conus distans, with a molecular mass of about 25 kDa, inhibits neurotransmitter release in rat hippocampus. This toxin (distans Toxin) inhibits the electrically evoked tritium labelled noradrenaline release from rat hippocamp...

journal_title：Neurochemistry international

authors： Saxena VK,Partoens P,de Block J,Coen EP,Vauquelin G,De Potter WP

更新日期：1992-01-01 00:00:00

abstract：:Concanavalin A-binding glycoprotein with 250 K M(r) found in the postsynaptic density (PSD)-enriched preparation (or synaptic cytoskeleton) from rat cerebellum was identified with P400 protein from the physicochemical properties and enrichment in the cerebellum. Proteins homologous to the cerebellar 250 K M(r) protein...

journal_title：Neurochemistry international

authors： Suzuki T,Abe-Dohmae S,Tanaka R

更新日期：1992-01-01 00:00:00

abstract：:Ketone bodies serve as a source of energy and as precursors for lipid synthesis in developing brain. Neurons, astrocytes and oligodendroglia all have the necessary enzymes for metabolizing ketone bodies. In this in vivo study it was found that all three cell types were able to use ketone bodies as precursors for the s...

journal_title：Neurochemistry international

authors： Poduslo SE,Miller K

更新日期：1991-01-01 00:00:00

abstract：:Phenylalanine and its phenyl metabolites produced a clear inhibition of chick brain mevalonate 5-pyrophosphate decarboxylase, while mevalonate kinase and mevalonate 5-phosphate kinase were not significantly affected. Phenolic derivatives produced a similar or higher inhibition than that found in the presence of phenyl...

journal_title：Neurochemistry international

authors： Castillo M,Iglesias J,Zafra MF,Garcia-Peregrin E

更新日期：1991-01-01 00:00:00

abstract：:Sprague-Dawley rats were made chronically vitamin D deficient (VDD) and hypocalcemic, or VDD and normocalcemic. Rickets, severely reduced body weight, hair shedding, lethargy, muscular paralysis and a high mortality rate are characteristic features of the male VDD/hypocalcemic animals. An assessment was made of the ne...

journal_title：Neurochemistry international

authors： Tenenhouse A,Warner M,Commissiong JW

更新日期：1991-01-01 00:00:00

abstract：:Changes in the level of ornithine decarboxylase mRNA after partial mesodiencephalic hemitransection were evaluated in various regions of the rat brain by means of in situ hybridization histochemistry coupled with computer-assisted image analysis. On days 1 and 2 after the lesion, increased accumulation of ornithine de...

journal_title：Neurochemistry international

authors： Zoli M,Bettuzzi S,Ferraguti F,Ingletti MC,Zini I,Fuxe K,Agnati LF,Corti A

更新日期：1991-01-01 00:00:00

abstract：:Long-term (12 h) incubation of NCB-20 neuroblastoma x Chinese hamster brain cell hybrid cells with phorbol dibutylate caused a decrease in the enzyme activity of protein kinase C (PKC). Under these conditions, the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] and the mobilization of intracellular Ca(2+) t...

journal_title：Neurochemistry international

authors： Okano Y,Higashida H,Tao F,Sakai T,Nozawa Y

更新日期：1991-01-01 00:00:00

abstract：:Fluorescence measurements and singlet singlet energy transfer experiments on endothelin-1 provide information on the conformation of this peptide in dilute aqueous solution. The tyrosine fluorescence quantum yield in the absence of transfer (in [Phe(21)]endothelin-1) is relatively large (?(tyr) = 0.39), indicating the...

journal_title：Neurochemistry international

authors： Pelton JT

更新日期：1991-01-01 00:00:00

abstract：:The cardiovascular and pulmonary responses to vasoactive intestinal contractor (VIC) were compared with those of endothelin (ET)-1, ET-2, ET-3 and sarafotoxin 6b (S6b) and the mechanism by which ET-1 alters vascular resistance was investigated in the hindquarters vascular bed of the cat. In a manner similar to ET-1 an...

journal_title：Neurochemistry international

authors： Minkes RK,Kadowitz PJ

更新日期：1991-01-01 00:00:00

abstract：:The less active form of human tyrosine hydroxylase has been previously reported but its physiological role is unknown. We partially purified the less active form of tyrosine hydroxylase from human adrenals, and examined differences in the properties of the active and less active forms. We succeeded in activation of th...

journal_title：Neurochemistry international

authors： Ishii A,Kiuchi K,Matsuyama M,Satake T,Nagatsu T

更新日期：1990-01-01 00:00:00

abstract：:The morphological differentiation of normal rat glioblasts induced by glia maturation factor (GMF) was investigated with regard to the reorganization of the cytoskeletal structures by the use of indirect immunofluorescence staining methods. The microfilaments were reorganized from a stress fiber form to a plasmalemmal...

journal_title：Neurochemistry international

authors： Ito J,Kato T,Tanaka R

更新日期：1990-01-01 00:00:00

abstract：:In this report evidence is presented for a membrane-associated polypeptide that regulates ligand binding properties of a neuronal acetylcholine receptor. Removal of membrane-associated compounds reversibly increased the number of BGTX binding sites and decreased the number of binding sites for ACh in neuronal membrane...

journal_title：Neurochemistry international

authors： Benke D,Breer H

更新日期：1990-01-01 00:00:00

abstract：:Recent data indicate that BMY 7378 demonstrates high affinity, selectivity and low intrinsic activity at hippocampal 5-HT(1A) receptors, suggesting that BMY 7378 may represent the first selective 5-HT(1A) functional antagonist. The present study examined the agonist and antagonist properties of BMY 7378 at spinal cord...

journal_title：Neurochemistry international

authors： Zemlan FP,Zieleniewski-Murphy A,Maureen Murphy R,Behbehani MM

更新日期：1990-01-01 00:00:00

abstract：:The oligosaccharide chains attached to the multiple forms of acetylcholinesterase (AChE) in a fraction enriched in membranes of the sarcoplasmic reticulum and T-tubules of rabbit skeletal muscle were studied using lectins. By sequential extraction of the membranes with Triton X-100, two preparations of soluble enzyme ...

journal_title：Neurochemistry international

authors： Cánovas-Muñoz MD,Vidal CJ

更新日期：1990-01-01 00:00:00

abstract：:The roles of membrane depolarization and calcium influx in the regulation of retinal serotonin N-acetyltransferase (NAT) activity were investigated in low-density monolayer cultures of chick retinal cells, in which photoreceptors represented approximately 70% of the total cell population. NAT activity expressed by the...

journal_title：Neurochemistry international

authors： Avendano G,Butler BJ,Michael Iuvone P

更新日期：1990-01-01 00:00:00

abstract：:The rat pheochromocytoma cell line PC12 undergoes morphological differentiation into neurone-like cells when exposed to nerve growth factor (NGF). We found that in PC12 cells the enzymatic activity of choline-acetyltransferase and of tyrosine hydroxylase changed very little during NGF exposure, while tyrosine hydroxyl...

journal_title：Neurochemistry international

authors： Reber BF,Porzig H,Becker C,Reuter H

更新日期：1990-01-01 00:00:00

abstract：:The changes in intracellar Ca(2+)-concentration, which occur during stimulation of vascular smooth muscle cells with excitatory agonists, are discussed. The effects of these agonists on the membrane potential of these cells are indirect, and mainly due to an activation of K(+)- and Cl(?)-channels by the release of cal...

journal_title：Neurochemistry international

authors： Casteels R,Droogmans G,Missiaen L

更新日期：1990-01-01 00:00:00

abstract：:A simple procedure for isolating a fraction enriched in synaptic plasma membrane (SPM) vesicles from sheep brain cortex is described and is used in studying uptake and release of [(3)H]GABA. The crude synaptosomal fraction was subjected to osmotic shock and the lysate was subfractioned in a discontinuous density gradi...

journal_title：Neurochemistry international

authors： Gonçalves PP,Santos MS,Carvalho AP

更新日期：1990-01-01 00:00:00

abstract：:Following intravitreal injections of (35)S-amino acids to label axonally transported proteins, calpain I and II from the superior colliculi and the lateral geniculate bodies were isolated via hydrophobic interaction chromatography, anion exchange HPLC and sodium-dodecyl sulphate electrophoresis. Among the slowly trans...

journal_title：Neurochemistry international

authors： Nilsson E,Karlsson JO

更新日期：1990-01-01 00:00:00

abstract：:The turnover of phospholipids was compared in peripheral nerves of Trembler dysmelinating mutant and control mice, after intraperitoneal and local injection of labeled ethanolamine. In the mutant sciatic nerve, neurochemical analysis showed that [(14)C]ethanolamine is incorporated into EGP (ethanolamine glycerophospho...

journal_title：Neurochemistry international

authors： Do Thi NA,Bourre JM,Koenig HL,Trouillet V,Dumont O,Piciotti M

更新日期：1990-01-01 00:00:00

abstract：:In the present study we investigated the effects of a single injection of reserpine on dopamine receptor function in rat striatum. The measurement of adenylate cyclase activity indicated that cAMP formation induced by the selective D-1 agonist SKF 82526 was greatly increased 2 and 20 h after reserpine; in contrast, th...

journal_title：Neurochemistry international

authors： Liberini P,Nisoli E,Missale C,Memo M,Buonamici M,Rossi A,Spano PF

更新日期：1989-01-01 00:00:00

abstract：:Marked age-related changes are noted in the neurotensin distribution of the human hippocampus. Different neuronal structures containing neurotensin are detected by immunohistochemistry during postnatal brain growth from birth to 4 years but no later. They are the neurotensin-immunoreactive pyramidal cells of the subic...

journal_title：Neurochemistry international

authors： Lotstra F,Mailleux P,Schiffmann SN,Vierendeels G,Vanderhaeghen JJ

更新日期：1989-01-01 00:00:00

abstract：:High-affinity binding sites for tritiated hemicholinium-3, a potent and selective inhibitor of the sodium-dependent high-affinity choline transport, have been found in synaptosomal membranes from insect ganglia. The binding of hemicholinium to ganglionic membrane preparations was saturable and specific with an apparen...

journal_title：Neurochemistry international

authors： Knipper M,Krieger J,Breer H

更新日期：1989-01-01 00:00:00

abstract：:This paper describes the background in experiment and theory on which present-day studies of the biochemistry of learning and memory are based. It then reviews specifically the use of passive avoidance training in the day-old chick as a model for the analysis of the cellular events associated with memory-formation. It...

journal_title：Neurochemistry international

authors： Rose SP

更新日期：1989-01-01 00:00:00

abstract：:Intact pieces or homogenates of rabbit retina were exposed to various established or putative retinal neurotransmitters for the study of receptors (or receptor-subtypes) linked to the production of cAMP. Since a dopamine-sensitive adenylate cyclase has been previously characterized in the retina of several species, th...

journal_title：Neurochemistry international

authors： Schorderet M

更新日期：1989-01-01 00:00:00

abstract：:For elucidation of thyroid hormone-induced responsiveness of fish brain, various doses (0.012, 0.025, 0.05, 0.1, 0.25, 0.5, 1, 2 and 4 ?g/g) of triiodothyronine (T(3)) were injected in Singi fish, Heteropneustes fossilis (Bloch), for 3 consecutive days and the changes in cytosolic NADP-dependent malic enzyme (ME, EC 1...

journal_title：Neurochemistry international

authors： Srabani De AK,Ray DA,Medda AK

更新日期：1989-01-01 00:00:00

abstract：:Sodium- and energy-dependent accumulation of [(3)H]l-glutamic acid (Glu) into rat cerebral cortical slices was inhibited by relatively high concentrations (40-100 ?M) of calmodulin antagonists, such as N-(6-aminohexyl)-1-naphthalenesulfonamide and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, in concentration-de...

journal_title：Neurochemistry international

authors： Yoneda Y,Ogita K,Yamada K,Kouda T,Ohgaki T,Uchida S,Meguri H

更新日期：1989-01-01 00:00:00

abstract：:The effects of neonatal X-irradiation on cerebellar cathecholamine levels in rats were studied at different postnatal intervals. Since synaptogenesis in the cerebellar cortex is basically a postnatal phenomenon, changes in noradrenaline (NA) and dopamine (DA) levels induced by X-rays on the cerebella (CE) of adult rat...

journal_title：Neurochemistry international

authors： Dopico AM,Zieher LM,Mayo J,Altschüller N,López JJ,Jaim-Etcheverry G

更新日期：1989-01-01 00:00:00

abstract：:The cholinergic nerve terminals of Torpedo electric organ are a rich source of a hydrophobic protein, the mediatophore, which endows artificial membranes with a calcium dependent mechanism that translocates acetylcholine. An antiserum raised against purified mediatophore permitted the identification of two different p...

journal_title：Neurochemistry international

authors： Morel N,Israel M,Synguelakis M,Le Gal la Salle G

更新日期：1989-01-01 00:00:00

abstract：:The effects of thyroid deficiency on the activity of glutamine synthetase (GS) and the number of glial fibrillary acidic (GFA) protein containing cells (the markers for astroglial cells) and on the 2?, 3?-cyclic nucleotide 3?-phosphohydrolase (CNPase) activity (the marker for oligodendroglial cells) were studied in th...

journal_title：Neurochemistry international

authors： Patel AJ,Hunt A,Kiss J

更新日期：1989-01-01 00:00:00

abstract：:The effect of the N-methylisoquinolinium ion (NMIQ(+)) on the activity of enzymes related to metabolism of dopamine was studied using a rat clonal pheochromocytoma PC12h cell line. The activities of tyrosine hydroxylase (TH), aromatic l-amino acid decarboxylase (AADC) and monoamine oxidase (MAO) were inhibited by NMIQ...

journal_title：Neurochemistry international

authors： Naoi M,Takahashi T,Parvez H,Kabeya R,Taguchi E,Yamaguchi K,Hirata Y,Minami M,Nagatsu T

更新日期：1989-01-01 00:00:00

abstract：:A protein, designated as protein 10 (MW = 29 kDa, pI = 5.3), was shown to be highly localized to the cochlear nuclei of guinea-pigs and rats by the use of two-dimensional gel electrophoresis. In gels from guinea-pig brain, this protein appeared greatest in amounts in the ventral cochlear nucleus and within the deep re...

journal_title：Neurochemistry international

authors： Winsky L,Nakata H,Jacobowitz DM

更新日期：1989-01-01 00:00:00

abstract：:GM1 ganglioside, after intravenous injection into rats, is absorbed and taken up by various organs and tissues, including brain. The capacity of brain to take up gangliosides, referred to weight unit, is comparable to that of kidney and muscle. After injection of [Gal-(3)H]GM1 a relevant portion of brain associated ra...

journal_title：Neurochemistry international

authors： Ghidoni R,Fiorilli A,Trinchera M,Venerando B,Chigorno V,Tettamanti G

更新日期：1989-01-01 00:00:00